2QGN
Crystal structure of tRNA isopentenylpyrophosphate transferase (BH2366) from Bacillus halodurans, Northeast Structural Genomics Consortium target BhR41.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97930 |
Spacegroup name | P 61 |
Unit cell lengths | 50.855, 50.855, 204.697 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.990 - 2.400 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.27700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.990 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.103 | 0.391 |
Number of reflections | 21615 | |
<I/σ(I)> | 18.3 | 4.09 |
Completeness [%] | 94.8 | 100 |
Redundancy | 8.9 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 7.5 | 291 | Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 20 micro-g/ml Subtilisin, 5 mM DTT. Reservoir solution: 16% PEG 3350, 200 mM Ammonium tartrate, MICROBATCH UNDER OIL, temperature 291K |