2QG9

Structure of a regulatory subunit mutant D19A of ATCase from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-10-13
Detector	UCSD MARK II
Wavelength(s)	1.5418
Spacegroup name	P 3 2 1
Unit cell lengths	122.290, 122.290, 142.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 2.700
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	XPLOR 3.1
RMSD bond length	0.017
RMSD bond angle	2.200
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.000
High resolution limit [Å]	2.700
Rmerge	0.101
Number of reflections	28103
Completeness [%]	91.3
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	5.7	298	0.1M citrate buffer, pH 5.7, MICRODIALYSIS, temperature 298.0K