2QG5
Cryptosporidium parvum calcium dependent protein kinase cgd7_1840
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.178, 86.476, 87.965 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 38.870 - 2.300 |
| R-factor | 0.22356 |
| Rwork | 0.220 |
| R-free | 0.28588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yrp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.351 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | |
| Number of reflections | 38421 | |
| <I/σ(I)> | 12.4 | 1.5 |
| Completeness [%] | 88.4 | 60.7 |
| Redundancy | 3.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.5M Na formate, 0.2M Bis Tris propane pH7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
