2QG3
CRYSTAL STRUCTURE OF A TYW3 METHYLTRANSFERASE-LIKE PROTEIN (AF_2059) FROM ARCHAEOGLOBUS FULGIDUS DSM 4304 AT 1.95 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979105 |
Spacegroup name | P 42 |
Unit cell lengths | 59.334, 59.334, 109.102 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.628 - 1.950 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.25300 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.495 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.628 | 28.628 | 2.060 |
High resolution limit [Å] | 1.950 | 6.170 | 1.950 |
Rmerge | 0.088 | 0.022 | 0.756 |
Total number of observations | 3247 | 14992 | |
Number of reflections | 27454 | ||
<I/σ(I)> | 7.5 | 21.7 | 1 |
Completeness [%] | 99.8 | 98.8 | 99.8 |
Redundancy | 3.7 | 3.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | NANODROP, 0.09M Citric acid, 0.01M Trisodium citrate, 20% MPD, Additive: 0.01 M Phenol, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 4.0 |
2 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 293 | NANODROP, 0.08M Citric acid, 0.02M Trisodium citrate, 22% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 4.1 |