2QG1
Crystal structure of the 11th PDZ domain of MPDZ (MUPP1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9182 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.409, 36.919, 63.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.800 - 1.400 |
R-factor | 0.17252 |
Rwork | 0.169 |
R-free | 0.23418 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2HE2 1tp3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.489 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.800 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.073 | 0.394 |
Number of reflections | 15501 | |
<I/σ(I)> | 13.5 | 2.6 |
Completeness [%] | 96.6 | 94.7 |
Redundancy | 4.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 0.8M (NH4)2SO4, 0.1M Citrate pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |