2QFW
Crystal structure of Saccharomyces cerevesiae mitochondrial NADP(+)-dependent isocitrate dehydrogenase in complex with isocitrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.699, 97.886, 190.651 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lwd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.890 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (7.1SSI) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.228 |
| Total number of observations | 22349 | |
| Number of reflections | 82166 | |
| <I/σ(I)> | 9 | 2 |
| Completeness [%] | 98.4 | 99.9 |
| Redundancy | 2.62 | 2.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1M HEPES-Na, 0.2M Li2SO4, 0.1M NaF, 20% PEG 4000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
