2QFR
Crystal structure of red kidney bean purple acid phosphatase with bound sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541 |
| Spacegroup name | I 41 |
| Unit cell lengths | 148.030, 148.030, 160.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.000 - 2.400 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kbp chain b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.115 | 0.302 |
| Number of reflections | 55092 | |
| <I/σ(I)> | 7.1 | 2.1 |
| Completeness [%] | 82.6 | 52 |
| Redundancy | 2.76 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 2M Ammonium sulfate, 0.1 M acetate pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
