2QFE
Distal C2-Like Domain of Human Calpain-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.513, 57.513, 88.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.990 - 1.450 |
| R-factor | 0.18848 |
| Rwork | 0.187 |
| R-free | 0.21541 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 27159 | |
| <I/σ(I)> | 56.3 | 3.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14.2 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | The crystals were grown in 1.4 M NaKPO4 pH 7.0, by mixing equal volumes of the precipitant solution and 13 mg/mL protein in binding buffer (500 mM NaCl, 50 mM Tris pH 8.0). The crystal was soaked for 1 hour in a solution containing 2 M NaBr., VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
