2QFC

Crystal Structure of Bacillus thuringiensis PlcR complexed with PapR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.976
Spacegroup name	P 61
Unit cell lengths	85.680, 85.680, 189.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.120 - 2.600
R-factor	0.228
Rwork	0.224
R-free	0.30400
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.690
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.200	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.078	0.457
Total number of observations		19692
Number of reflections	24242
<I/σ(I)>	5.6	1.6
Completeness [%]	100.0	100
Redundancy	8.2	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	40% PEG 200, 100 mM MES, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	40% PEG 200, 100 mM MES, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	40% PEG 200, 100 mM MES, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K