2QF4
High resolution structure of the major periplasmic domain from the cell shape-determining filament MreC (orthorhombic form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.247, 70.362, 89.763 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.385 - 1.200 |
R-factor | 0.145 |
Rwork | 0.144 |
R-free | 0.16700 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.674 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.385 | 1.260 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.057 | 0.472 |
Number of reflections | 99707 | |
<I/σ(I)> | 8.3 | 1.6 |
Completeness [%] | 99.7 | 98.8 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M Na Acetate pH 4.6, 0.2M Ammonium sulfate, 12.5% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |