2QEZ

Crystal structure of ethanolamine ammonia-lyase heavy chain (YP_013784.1) from Listeria monocytogenes 4b F2365 at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97925, 0.97895
Spacegroup name	P 21 21 2
Unit cell lengths	188.330, 223.700, 65.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 2.150
R-factor	0.23
Rwork	0.228
R-free	0.27900
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.594
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.210	29.210	2.170
High resolution limit [Å]	2.150	4.510	2.150
Rmerge	0.054	0.019	0.487
Number of reflections	162506	30960	27770
<I/σ(I)>	9.26	32.2	1.5
Completeness [%]	98.9	97.4	94.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 30.0% 2-methyl-2,4-pentanediol, 0.2M Magnesium acetate, 0.1M Sodium pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K