2QEY
Rat cytosolic PEPCK in complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.713, 118.851, 60.704 |
| Unit cell angles | 90.00, 108.84, 90.00 |
Refinement procedure
| Resolution | 32.620 - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qew |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.117 | 0.054 | 0.575 |
| Number of reflections | 42128 | ||
| <I/σ(I)> | 10.5 | ||
| Completeness [%] | 89.3 | 99.1 | 46.1 |
| Redundancy | 6.7 | 7.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 25% PEG 3350, 0.1M HEPES, pH 7.4, 10mM MnCl2, 10mM GTP, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
