2QEW
Rat cytosolic PEPCK, in complex with manganese ion.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-04-28 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.448, 119.046, 60.599 |
Unit cell angles | 90.00, 109.59, 90.00 |
Refinement procedure
Resolution | 24.260 - 1.800 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1khg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.079 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.546 |
Number of reflections | 53469 | |
<I/σ(I)> | 13.1 | |
Completeness [%] | 97.9 | 93.2 |
Redundancy | 7.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 12-30% PEG 3350, 0.1M HEPES pH 7.4, 10 mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |