2QEV

Crystal Structure of Anopheles gambiae D7r4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791533
Spacegroup name	P 43
Unit cell lengths	62.651, 62.651, 42.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.620 - 1.998
R-factor	0.22
Rwork	0.219
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.060
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.620	2.070
High resolution limit [Å]	1.998	1.998
Rmerge	0.039	0.303
Number of reflections	11190
<I/σ(I)>	18.3	3.3
Completeness [%]	97.8	84.3
Redundancy	4.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	20 % PEG 6000, 0.1 M Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	8	298	20 % PEG 6000, 0.1 M Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K