2QET

Structure of the mutant S211A of the ribosome inactivating protein PDL4 from P. dioica in complex with adenine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-08
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	43.619, 61.541, 91.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.240
Rwork	0.122
R-free	0.15500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qcg
RMSD bond length	0.029
RMSD bond angle	0.037
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	IlMilione
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.240	1.240
Number of reflections	68965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			VAPOR DIFFUSION