2QEP
Crystal structure of the D1 domain of PTPRN2 (IA2beta)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99990 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 131.541, 136.555, 35.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.230 - 2.500 |
| R-factor | 0.2389 |
| Rwork | 0.236 |
| R-free | 0.28601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i1y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.230 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.139 | 0.465 |
| Number of reflections | 23096 | |
| <I/σ(I)> | 9.1 | 1.9 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M Na/KPO4, 0.1 M Bis-tris-propane pH 6.5, 20.0% PEG 3350, 10.0% Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
