2QEN
The walker-type atpase paby2304 of pyrococcus abyssi
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.2836,0.849972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.614, 49.195, 108.863 |
| Unit cell angles | 90.00, 98.10, 90.00 |
Refinement procedure
| Resolution | 28.300 - 2.250 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.314 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.043 | 0.120 |
| Number of reflections | 18280 | |
| Completeness [%] | 95.0 | 91.6 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEG 3350, PH 6.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 297K, pH 6.50 |
