2QEJ

Crystal structure of a Staphylococcus aureus protein (SSL7) in complex with Fc of human IgA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-05-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	71.306, 109.263, 170.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.820 - 3.200
Rwork	0.229
R-free	0.31200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1OW0 and 1V1P
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.820	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	22643
<I/σ(I)>	10	2.8
Completeness [%]	98.0	97
Redundancy	6.3	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	12% PEG 8000, 66 mM sodium cacodylate pH 6.5, 130 mM calcium acetate, VAPOR DIFFUSION, temperature 291K