2QE7
Crystal structure of the f1-atpase from the thermoalkaliphilic bacterium bacillus sp. ta2.a1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-03-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979540 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.210, 173.020, 218.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.997 - 3.060 |
| Rwork | 0.252 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bmf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.662 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.250 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.119 | 0.716 |
| Number of reflections | 90041 | |
| <I/σ(I)> | 10.2 | 1.8 |
| Completeness [%] | 91.1 | 74.9 |
| Redundancy | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.8 | 296 | 1M LiCl, 0.1M Tris-HCl, 20% PEG 6000 , pH 8.8, MICRO-BATCH, temperature 296K |
