2QDO
NblA protein from T. vulcanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.190, 42.390, 50.560 |
| Unit cell angles | 69.00, 83.36, 61.25 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| Rwork | 0.248 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q8v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.610 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.090 |
| Number of reflections | 9900 |
| <I/σ(I)> | 19.5 |
| Completeness [%] | 96.7 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 25% ethylene glycol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
