2QDB

Crystal structure of staphylococcal nuclease variant E75Q/D21N/T33V/T41I/S59A/P117G/S128A at 100 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-08-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41
Unit cell lengths	48.668, 48.668, 63.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.700 - 2.200
R-factor	0.26
Rwork	0.239
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1EZ6 modified as follows: all variant residues were truncated to Ala B's were reset to 20.0 A^2 waters were removed
Data reduction software	CrystalClear
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	7518
<I/σ(I)>	17.4	5.2
Completeness [%]	99.3	98.6
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	38% MPD and 25 mM potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K