2QCW
Crystal Structure of Bone Morphogenetic Protein-6 (BMP-6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-31 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.446, 97.446, 87.377 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.500 - 2.490 |
| R-factor | 0.23486 |
| Rwork | 0.233 |
| R-free | 0.27589 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BMP-7 ligand monomer taken from PDB entry 1lxi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.396 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.053 | 0.437 |
| Number of reflections | 16679 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 93.0 | 57.2 |
| Redundancy | 5.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 15% 2-methyl-2,4-pentanediol (MPD), 0.2 M Tri-Na Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
