2QCU
Crystal structure of Glycerol-3-phosphate Dehydrogenase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9803 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 113.791, 114.097, 192.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.750 |
R-factor | 0.20564 |
Rwork | 0.204 |
R-free | 0.23998 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.740 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.061 | 0.033 | 0.712 |
Number of reflections | 136571 | ||
<I/σ(I)> | 11.8 | ||
Completeness [%] | 99.4 | 98.9 | 98.1 |
Redundancy | 9 | 9.4 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M di-Ammonium hydrogen phosphate, 0.1 M Tris-HCl pH 8.5, 12 % w/v PEG MME 550, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M di-Ammonium hydrogen phosphate, 0.1 M Tris-HCl pH 8.5, 12 % w/v PEG MME 550, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M di-Ammonium hydrogen phosphate, 0.1 M Tris-HCl pH 8.5, 12 % w/v PEG MME 550, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |