2QCQ
Crystal structure of Bone Morphogenetic Protein-3 (BMP-3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11 |
| Spacegroup name | H 3 |
| Unit cell lengths | 96.850, 96.850, 101.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.960 - 2.210 |
| R-factor | 0.22956 |
| Rwork | 0.228 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BMP-2 ligand monomer from PDB entry 2goo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.460 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.313 |
| Number of reflections | 17488 | |
| <I/σ(I)> | 37.9 | 6.2 |
| Completeness [%] | 97.9 | 94.4 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 296 | 26% tert-butanol and 0.1 M Na Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
