2QC8
Crystal structure of human glutamine synthetase in complex with ADP and methionine sulfoximine phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.210, 126.080, 188.170 |
Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
Resolution | 39.940 - 2.600 |
R-factor | 0.168 |
Rwork | 0.166 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ojw |
RMSD bond length | 0.015 |
RMSD bond angle | 1.592 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.120 | 0.526 |
Number of reflections | 129787 | |
<I/σ(I)> | 15.6 | 4.1 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.5 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | 1.1M Sodium malonate, 0.5% Jeffamine ED-2001, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 7.00 |