2QC5
Streptogramin B lyase structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-19 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.465, 68.117, 73.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.800 |
| R-factor | 0.18037 |
| Rwork | 0.179 |
| R-free | 0.21208 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.303 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.060 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.039 | 0.139 |
| Number of reflections | 27635 | |
| <I/σ(I)> | 16.9 | 5.5 |
| Completeness [%] | 96.6 | 92.8 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277 | 25mM HEPES; 100mM CaCl2;12,5% PEG3350, 5mM L-cysteine , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.50 |
