2QB8
Saccharomyces cerevisiae cytosolic exopolyphosphatase, ATP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.134, 82.847, 121.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.500 - 1.900 |
| R-factor | 0.18633 |
| Rwork | 0.184 |
| R-free | 0.23287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qb6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 60043 | |
| <I/σ(I)> | 7.7 | 1.6 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 7% PEG 4000, 150 mM Ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
