2QB6
Saccharomyces cerevisiae cytosolic exopolyphosphatase, sulfate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.660, 82.914, 119.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.400 - 1.800 |
| R-factor | 0.15944 |
| Rwork | 0.157 |
| R-free | 0.20099 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.467 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.400 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 64994 | |
| <I/σ(I)> | 8.5 | 3.7 |
| Completeness [%] | 88.2 | 51.4 |
| Redundancy | 4.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 7% PEG 4000, 150 mM (NH4)2SO4, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
