2QAC

The closed MTIP-MyosinA-tail complex from the malaria parasite invasion machinery

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98000
Spacegroup name	P 21 21 21
Unit cell lengths	37.217, 54.120, 76.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.580 - 1.700
R-factor	0.16984
Rwork	0.167
R-free	0.22828
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.942
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.791
High resolution limit [Å]	1.700	1.700
Number of reflections	14110
<I/σ(I)>	15.2	1.8
Completeness [%]	100.0	100
Redundancy	5.1	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20% PEG 3350, 0.2 M KNO3, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20% PEG 3350, 0.2 M KNO3, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20% PEG 3350, 0.2 M KNO3, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K