2QA7

Crystal structure of Huntingtin-interacting protein 1 (HIP1) coiled-coil domain with a basic surface suitable for HIP-protein interactor (HIPPI)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-07
Detector	NOIR-1
Wavelength(s)	0.96407, 0.97907, 0.97892
Spacegroup name	P 43
Unit cell lengths	72.900, 72.900, 106.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.32
Rwork	0.265
R-free	0.32400
Structure solution method	MAD
Starting model (for MR)	none
RMSD bond length	0.009
RMSD bond angle	1.190
Data reduction software	d*TREK
Data scaling software	d*TREK (9.7LDz)
Phasing software	RESOLVE (2.03)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.390	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.093	0.488
Total number of observations		10273
Number of reflections	15320
<I/σ(I)>	10.3	2.9
Completeness [%]	99.8	99.9
Redundancy	7.07	6.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	15% PEG 3350, 0.1 M succinate, 0.2 M potassium sodium tartrate, 0.01 M nickle chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K