2QA0

Structure of Adeno-Associated virus serotype 8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9175
Spacegroup name	P 63 2 2
Unit cell lengths	254.840, 254.840, 445.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.600
Rwork	0.255
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lp3
RMSD bond length	1.540
RMSD bond angle	0.007
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	202494
<I/σ(I)>	11.7	2.4
Completeness [%]	78.2	62.2
Redundancy	2.2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1M NaCl, 4% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1M NaCl, 4% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K