2Q9U
Crystal structure of the flavodiiron protein from Giardia intestinalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 111.967, 115.059, 67.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.16664 |
| Rwork | 0.165 |
| R-free | 0.20409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ycf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.393 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.430 |
| Number of reflections | 69601 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 98.3 | 92.3 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 16% PEG 3350, 0.2 M potassium nitrate,0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
