2Q9D
Structure of spin-labeled T4 lysozyme mutant A41R1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.808, 59.808, 94.895 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.400 |
R-factor | 0.1517 |
Rwork | 0.152 |
R-free | 0.20190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lzm |
RMSD bond length | 0.012 |
RMSD bond angle | 0.028 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 38697 | |
<I/σ(I)> | 23.4 | 5.3 |
Completeness [%] | 98.4 | 97.4 |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 294 | 2.0 M Na/K Phosphate, 240 mM NaCl, 40 mM 2-hydroxyethyl disulfide, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |