2Q99

Crystal Structure of Saccharopine Dehydrogenase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9200,0.9794,0.9796,1.0080
Spacegroup name	P 21 21 21
Unit cell lengths	64.680, 74.920, 74.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.970 - 1.640
Rwork	0.187
R-free	0.21600
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.08)
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	1.700
High resolution limit [Å]	1.640	1.640
Rmerge	0.073	0.699
Number of reflections	44946
<I/σ(I)>	11.3
Completeness [%]	98.8	89.3
Redundancy	6.7	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K