2Q96
E. coli methionine aminopeptidase Mn-form with inhibitor A18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.285, 62.028, 52.426 |
Unit cell angles | 90.00, 108.76, 90.00 |
Refinement procedure
Resolution | 19.936 - 1.600 |
Rwork | 0.211 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xnz |
RMSD bond length | 0.004 |
RMSD bond angle | 1.305 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.936 | 19.940 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.038 | 0.026 | 0.138 |
Total number of observations | 3646 | 15554 | |
Number of reflections | 31502 | ||
<I/σ(I)> | 15.2 | 24.5 | 4.9 |
Completeness [%] | 99.9 | 98.2 | 99.6 |
Redundancy | 3.6 | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 10-15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K |