2Q95
E. coli methionine aminopeptidase Mn-form with inhibitor A05
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.847, 62.373, 52.422 |
| Unit cell angles | 90.00, 108.39, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.214 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xnz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.327 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 23.966 | 23.970 | 1.790 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.055 | 0.050 | 0.389 |
| Total number of observations | 3028 | 13105 | |
| Number of reflections | 26219 | ||
| <I/σ(I)> | 9.2 | 10.4 | 1.8 |
| Completeness [%] | 100.0 | 98.6 | 100 |
| Redundancy | 3.6 | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 10-15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K |
