2Q94
E. coli methionine aminopeptidase Mn-form with inhibitor A04
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.199, 60.605, 50.624 |
| Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
| Resolution | 19.910 - 1.630 |
| Rwork | 0.213 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xnz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.322 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.913 | 19.910 | 1.720 |
| High resolution limit [Å] | 1.630 | 5.150 | 1.630 |
| Rmerge | 0.044 | 0.034 | 0.363 |
| Total number of observations | 3165 | 13929 | |
| Number of reflections | 27719 | ||
| <I/σ(I)> | 12.1 | 15.3 | 2 |
| Completeness [%] | 99.3 | 98.2 | 98 |
| Redundancy | 3.6 | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 10-15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K |
