2Q92
E. coli methionine aminopeptidase Mn-form with inhibitor B23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.137, 60.984, 50.693 |
| Unit cell angles | 90.00, 104.93, 90.00 |
Refinement procedure
| Resolution | 26.400 - 1.900 |
| Rwork | 0.203 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xnz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.318 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.400 | 29.660 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.054 | 0.041 | 0.221 |
| Total number of observations | 2016 | 9045 | |
| Number of reflections | 17799 | ||
| <I/σ(I)> | 11.7 | 13.6 | 3.4 |
| Completeness [%] | 100.0 | 98.7 | 100 |
| Redundancy | 3.6 | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 10-15% PEG 20000, 0.1 M MES (pH 6.5), vapor diffusion, hanging drop, temperature 291K |
