2Q8T
Crystal Structure of the CC chemokine CCL14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.345, 78.073, 59.639 |
| Unit cell angles | 90.00, 121.65, 90.00 |
Refinement procedure
| Resolution | 30.950 - 2.230 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b3a |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.685 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.039 | 0.100 |
| Number of reflections | 17814 | |
| <I/σ(I)> | 30.98 | 14.3 |
| Completeness [%] | 98.9 | 99.2 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.2 M sodium formate, 20% w/v PEG 3350, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
