2Q8L
Crystal structure of orotidine 5'-phosphate decarboxylase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.563, 70.909, 70.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.950 - 2.100 |
| R-factor | 0.21746 |
| Rwork | 0.216 |
| R-free | 0.24929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f84 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.464 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.950 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.267 |
| Number of reflections | 19423 | |
| <I/σ(I)> | 11.8 | 7.39 |
| Completeness [%] | 97.2 | 100 |
| Redundancy | 9.3 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 15% PEG 10000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
