2Q8A

Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	189.762, 51.203, 91.518
Unit cell angles	90.00, 111.09, 90.00

Refinement procedure

Resolution	38.920 - 2.400
R-factor	0.20433
Rwork	0.202
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z40 2cgr
RMSD bond length	0.008
RMSD bond angle	1.138
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	30851
<I/σ(I)>	13	4.7
Completeness [%]	99.9	99.9
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	20 MM MES, 10 MM MNCL2, 6 % PEG 3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K