2Q82
Crystal structure of core protein P7 from Pseudomonas phage phi12. Northeast Structural Genomics Target OC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97947 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 75.499, 75.499, 46.486 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.830 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.22600 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.050 | 0.031 | 0.674 |
Number of reflections | 29155 | ||
<I/σ(I)> | 12 | ||
Completeness [%] | 89.4 | 98.4 | 48.6 |
Redundancy | 10.5 | 11.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% PEG10000, 100mM Sodium acetate, 10% n-Hexyl-D-glucoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K |