2Q7G
Pyrrolysine tRNA Synthetase bound to a pyrrolysine analogue (cyc) and ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97917 |
Spacegroup name | P 64 |
Unit cell lengths | 104.773, 104.773, 71.594 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 1.900 |
R-factor | 0.16374 |
Rwork | 0.162 |
R-free | 0.18943 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.282 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.078 | 0.328 |
Number of reflections | 33104 | |
<I/σ(I)> | 29.3 | 5.2 |
Completeness [%] | 99.6 | 96.5 |
Redundancy | 10.8 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 289 | 100 mM Tris 10% PEG2000 MME, pH 7.5, VAPOR DIFFUSION, temperature 289K |