2Q7E
The structure of pyrrolysyl-tRNA synthetase bound to an ATP analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97917 |
Spacegroup name | P 64 |
Unit cell lengths | 105.124, 105.124, 70.314 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.400 - 1.800 |
R-factor | 0.16727 |
Rwork | 0.166 |
R-free | 0.19119 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.297 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.879 |
Number of reflections | 38485 | |
<I/σ(I)> | 48.2 | 2 |
Completeness [%] | 98.8 | 95.7 |
Redundancy | 10.6 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 289 | 100mM Tris 10% PEG2000 MME , pH 7.5, VAPOR DIFFUSION, temperature 289K |