2Q7E
The structure of pyrrolysyl-tRNA synthetase bound to an ATP analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 64 |
| Unit cell lengths | 105.124, 105.124, 70.314 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.400 - 1.800 |
| R-factor | 0.16727 |
| Rwork | 0.166 |
| R-free | 0.19119 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.297 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.879 |
| Number of reflections | 38485 | |
| <I/σ(I)> | 48.2 | 2 |
| Completeness [%] | 98.8 | 95.7 |
| Redundancy | 10.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 289 | 100mM Tris 10% PEG2000 MME , pH 7.5, VAPOR DIFFUSION, temperature 289K |
