2Q7A
Crystal structure of the cell surface heme transfer protein Shp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 65 |
Unit cell lengths | 82.317, 82.317, 106.054 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.170 - 2.100 |
R-factor | 0.166 |
Rwork | 0.163 |
R-free | 0.22200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.164 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.115 | 0.593 |
Number of reflections | 23738 | |
<I/σ(I)> | 20.4 | 2.3 |
Completeness [%] | 99.6 | 95.2 |
Redundancy | 10.8 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.8M ammonium sulfate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.8M ammonium sulfate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.8M ammonium sulfate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |