2Q73
Crystal structure of iMazG from Vibrio DAT 722: Ctag-iMazG (P41212)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.963, 87.963, 158.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.840 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q5z |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.263 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.966 | 1.900 |
| High resolution limit [Å] | 1.798 | 1.800 |
| Rmerge | 0.079 | 0.551 |
| Number of reflections | 55795 | |
| <I/σ(I)> | 3.9 | 1.4 |
| Completeness [%] | 94.9 | 100 |
| Redundancy | 8.7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.05 | 293 | 0.1 M sodium citrate, 1.9 M ammonium sulfate, 500 mM NaCl, 10% 2-methyl-2,4-pentanediol, 10 mM MgCl2, 10 mM dCTP, pH 5.05, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
