2Q6T

Crystal structure of the Thermus aquaticus DnaB monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1404
Spacegroup name	P 31 2 1
Unit cell lengths	104.707, 104.707, 363.258
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.243
Rwork	0.241
R-free	0.28453
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.139
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.974
High resolution limit [Å]	2.900	2.900
Rmerge	0.098
Number of reflections	47366
<I/σ(I)>	15.8	1.9
Completeness [%]	99.2	99.92
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	1.3M Ammonium Sulfate, 10mM Magnesium Sulfate, 5% 1,6-hexanediol, 100mM MES, pH 6.5, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	6.5	298	1.3M Ammonium Sulfate, 10mM Magnesium Sulfate, 5% 1,6-hexanediol, 100mM MES, pH 6.5, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	6.5	298	1.3M Ammonium Sulfate, 10mM Magnesium Sulfate, 5% 1,6-hexanediol, 100mM MES, pH 6.5, VAPOR DIFFUSION, temperature 298K