2Q6E
Crystal structure of glucuronate isomerase from Bacillus halodurans complexed with Zn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 133.372, 133.372, 196.821 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.990 - 2.400 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q08 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.067 |
Number of reflections | 64148 |
Completeness [%] | 92.0 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG 3350, 0.1M Tris-HCl, 0.2M Sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |