2Q6A
Crystal Structure of Nak channel D66E mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-13 |
Detector | SBC-3 |
Wavelength(s) | 0.9805 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.979, 85.630, 130.134 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
Rwork | 0.245 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ahz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.203 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.070 | 0.030 | 0.919 |
Number of reflections | 14211 | ||
<I/σ(I)> | 9.5 | ||
Completeness [%] | 97.1 | 97.2 | 96.9 |
Redundancy | 5.4 | 5.7 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 200 mM CaCl2, 100 mM Tris-HCl, 37-42% PEG400, 4% t-Butanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |