2Q5Y

Crystal Structure of the C-terminal domain of hNup98

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-18
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1
Spacegroup name	P 31 2 1
Unit cell lengths	90.301, 90.301, 97.648
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.300
Rwork	0.228
R-free	0.26000
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.156
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM (4.1)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.380
High resolution limit [Å]	2.300	4.960	2.300
Rmerge	0.058	0.029	0.299
Number of reflections	20739
<I/σ(I)>	10.1
Completeness [%]	98.1	95.6	97.6
Redundancy	2.7	2.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	296	0.1M Tris, 0.2M MgAc2, 22% PEG8000, microseeding, pH 8.1, vapor diffusion, hanging drop, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	8.1	296	0.1M Tris, 0.2M MgAc2, 22% PEG8000, microseeding, pH 8.1, vapor diffusion, hanging drop, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	8.1	296	0.1M Tris, 0.2M MgAc2, 22% PEG8000, microseeding, pH 8.1, vapor diffusion, hanging drop, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	8.1	296	0.1M Tris, 0.2M MgAc2, 22% PEG8000, microseeding, pH 8.1, vapor diffusion, hanging drop, temperature 296K