2Q5Y
Crystal Structure of the C-terminal domain of hNup98
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.301, 90.301, 97.648 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.300 |
Rwork | 0.228 |
R-free | 0.26000 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.156 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM (4.1) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.960 | 2.300 |
Rmerge | 0.058 | 0.029 | 0.299 |
Number of reflections | 20739 | ||
<I/σ(I)> | 10.1 | ||
Completeness [%] | 98.1 | 95.6 | 97.6 |
Redundancy | 2.7 | 2.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 296 | 0.1M Tris, 0.2M MgAc2, 22% PEG8000, microseeding, pH 8.1, vapor diffusion, hanging drop, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 296 | 0.1M Tris, 0.2M MgAc2, 22% PEG8000, microseeding, pH 8.1, vapor diffusion, hanging drop, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 296 | 0.1M Tris, 0.2M MgAc2, 22% PEG8000, microseeding, pH 8.1, vapor diffusion, hanging drop, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 296 | 0.1M Tris, 0.2M MgAc2, 22% PEG8000, microseeding, pH 8.1, vapor diffusion, hanging drop, temperature 296K |